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ea0029p1347 | Pituitary Basic | ICEECE2012

Inhibition of methylation stimulates POMC expression and ACTH secretion in human pituitary ACTH-secreting tumors

Cassarino M. , Sesta A. , Pagliardini L. , Losa M. , Lasio G. , Cavagnini F. , Pecori Giraldi F.

DNA methylation has recently been shown to play a role in postnatal as well as developmental gene regulation. Transfer and removal of methyl groups from CpG islands in the promoter region of specific genes has been associated with gene silencing or activation, respectively. POMC is known to present several methylation sites and demethylation of these sites has been shown to be linked to POMC expression by neuroendocrine tumors outside the pituitary.<p class="...

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ea0029p1352 | Pituitary Basic | ICEECE2012

Effects of the combined treatment with AMPK activator and somatostatin-14 on hormone secretion and cell proliferation in cultured GH-secreting pituitary tumor cells

Tulipano G. , Faggi L. , Losa M. , Mortini P. , Spinello M. , Cocchi D. , Giustina A.

AMP-activated protein kinase (AMPK) activation decreased GH release and intracellular GH storage in rat pituitary cell cultures and reduced cell proliferation in rat pituitary adenomatous cells (GH3) cultures.We investigated the effects of the AMPK activator 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) as compared to somatostatin-14 on cell viability (MTT assay after 48-h) and GH secretion (over 24 h) in 16 human GH-secreting pituitary adenomas ...

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Endocrine Abstracts

Inhibition of methylation stimulates POMC expression and ACTH secretion in human pituitary ACTH-secreting tumors

Effects of the combined treatment with AMPK activator and somatostatin-14 on hormone secretion and cell proliferation in cultured GH-secreting pituitary tumor cells

BiosciAbstracts